A study of the interaction of histones with DNA using isopycnic centrifugation in metrizamide gradients
Open Access
- 1 January 1975
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (5) , 723-731
- https://doi.org/10.1093/nar/2.5.723
Abstract
Isopycnic sedimentation in metrizamide gradients has shown that mouseliver histones bind co-operatively to both homologous and bacterial DNA's. However, at lon input ratios of histons to DNA, two types of stable complex are formed, depending on the histone concentration. One complex contains half as much histons as DNA while the other contains approximately equal amounts of histone and DNA. At high input ratios of histone to DNA extra histone is bound giving complexes containing up to twice as much histone as DNA. Poly-L-lysine and protamine were also found to bind co-operatively to DNA.Keywords
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