Helminthosporium maydis Race T Toxin Induces Leakage of NAD+ from T Cytoplasm Corn Mitochondria
- 1 June 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 63 (6) , 1149-1153
- https://doi.org/10.1104/pp.63.6.1149
Abstract
The mechanism by which H. maydis race T toxin inhibits respiration dependent on NAD+-linked substrates in T cytoplasm corn [Zea mays] mitochondria was investigated. The toxin did not cause leakage of the soluble matrix enzyme malate dehydrogenase from the mitochondria or inhibit malate dehydrogenase or isocitrate dehydrogenase directly. The toxin increased the permeability of the inner membranes of T cytoplasm, but not N cytoplasm, mitochondria to NAD+. Added NAD+ partially or fully restored toxin-inhibited electron transport in T cytoplasm mitochondria. Thiamin pyrophosphate had a similar effect when malate was the substrate. The inhibition of respiration of NAD+-linked substrates by the toxin is due to depletion of the intramitochondrial pool of NAD+ and other coenzymes.This publication has 16 references indexed in Scilit:
- Cytoplasm-specific Effects of Helminthosporium maydis Race T Toxin on Survival of Corn Mesophyll ProtoplastsPlant Physiology, 1978
- Pyruvate and Malate Transport and Oxidation in Corn MitochondriaPlant Physiology, 1977
- Reversible Effects of Toxin from Helminthosporium maydis Race T on Oxidative Phosphorylation by Mitochondria from MaizePlant Physiology, 1977
- The influence of osmolarity on the reduction of exogenous cytochrome c and permeability of the inner membrane of Jerusalem artichoke mitochondriaBiochemical Journal, 1974
- Isolation and Partial Characterization of Four Host-specific Toxins of Helminthosporium maydis (Race T)Plant Physiology, 1974
- Southern Corn Leaf Blight: Susceptible and Resistant MitochondriaScience, 1971
- The molar extinction coefficient of 2,6-dichlorophenol indophenolBiochimica et Biophysica Acta (BBA) - General Subjects, 1964
- Water Uptake and Extrusion by Mitochondria in Relation to Oxidative PhosphorylationPhysiological Reviews, 1962
- Mechanism of depletion of mitochondrial pyridine nucleotidesBiochimica et Biophysica Acta, 1960
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951