Helminthosporium maydis Race T Toxin Induces Leakage of NAD+ from T Cytoplasm Corn Mitochondria

Abstract
The mechanism by which H. maydis race T toxin inhibits respiration dependent on NAD+-linked substrates in T cytoplasm corn [Zea mays] mitochondria was investigated. The toxin did not cause leakage of the soluble matrix enzyme malate dehydrogenase from the mitochondria or inhibit malate dehydrogenase or isocitrate dehydrogenase directly. The toxin increased the permeability of the inner membranes of T cytoplasm, but not N cytoplasm, mitochondria to NAD+. Added NAD+ partially or fully restored toxin-inhibited electron transport in T cytoplasm mitochondria. Thiamin pyrophosphate had a similar effect when malate was the substrate. The inhibition of respiration of NAD+-linked substrates by the toxin is due to depletion of the intramitochondrial pool of NAD+ and other coenzymes.