STRUCTURAL ALTERATIONS IN CRYSTALLINE GLUTAMIC DEHYDROGENASE INDUCED BY STEROID HORMONES

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Abstract
Diethyl-stilbestrol, estradiol, and progesterone, all of which inhibit the glutamic dehydrogenase reaction, promote dissociation of the crystalline beef liver enzyme into subunits as determined by ultracentrifugation. Cortisone, inactive as an inhibitor, did not influence the behavior of the protein in the ultracentrifuge. ADP could prevent both the steroid inhibition and steroid-induced disaggregation of the enzyme. It is, therefore, suggested that the change in the structure of the enzyme induced by steroids is responsible for the action of the hormones as inhibitors of crystalline beef liver glutamic dehydrogenase.