Frequent loss of heterozygosity on chromosome 17 at 17q11.2-q12 in Barrett's adenocarcinoma
Open Access
- 1 May 1995
- journal article
- Published by Springer Nature in British Journal of Cancer
- Vol. 71 (5) , 995-998
- https://doi.org/10.1038/bjc.1995.191
Abstract
Allelic loss on chromosome 17 in 18 Barrett's oesophageal tumours was analysed with 17 polymorphic microsatellite markers. Loss of heterozygosity (LOH) of one or more markers was seen in 72% (13 of 18) tumours on 17p and 56% (10 of 18) on 17q. The highest 17p losses were found at D17S799 (62%, five of eight) and D17S261 (55%, five of nine), while loss at the p53 locus was 31% (5 of 16). The highest loss on 17q was found at the TCF-2 (17q11.2-q12) locus with 66% (8 of 12) LOH. TCF-2 was the only marker lost in two of the tumour samples; furthermore, TCF-2 was lost in four other tumours which retained heterozygosity at the markers on either side of it, D17S261 and D17S740. Six markers were used to assess LOH at 17q11.2-q12, and five of eight of the tumour specimens which had LOH at TCF-2 had no other loss on 17q. No statistically significant correlations were found between loss on 17q or 17p and any clinicopathological parameters. We propose from these data that the 17q11.2-q12 region contains a novel predisposing gene in Barrett's adenocarcinomas and may represent the site of a tumour-suppressor gene.Keywords
This publication has 28 references indexed in Scilit:
- BRCA1 Mutations in Primary Breast and Ovarian CarcinomasScience, 1994
- Barrett's oesophagus—cancer risk, biology and therapeutic managementAlimentary Pharmacology & Therapeutics, 1993
- Loss of heterozygosity involving the APC and MCC genetic loci occurs in the majority of human esophageal cancers.Proceedings of the National Academy of Sciences, 1992
- Assignment of the human prohibition gene (PHB) to chromosome 17 and identification of a DNA polymorphismGenomics, 1991
- Reduction to homozygosity involving p53 in esophageal cancers demonstrated by the polymerase chain reaction.Proceedings of the National Academy of Sciences, 1991
- Linkage of Early-Onset Familial Breast Cancer to Chromosome 17q21Science, 1990
- Frequent mutation of the p53 gene in human esophageal cancer.Proceedings of the National Academy of Sciences, 1990
- Evidence implicating at least two genes on chromosome 17p in breast carcinogenesisThe Lancet, 1990
- Neu-Protein Overexpression in Breast CancerNew England Journal of Medicine, 1988
- Genetic Alterations during Colorectal-Tumor DevelopmentNew England Journal of Medicine, 1988