Induction of Myelin Components: Cyclic AMP Increases the Synthesis Rate of 2',3'-Cyclic Nucleotide 3'-Phosphohydrolase in C6Glioma Cells
- 1 April 1985
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 44 (4) , 1242-1251
- https://doi.org/10.1111/j.1471-4159.1985.tb08750.x
Abstract
In an effort to determine the factors that stimulate myelin synthesis, we investigated the mechanism by which dibutyryl cyclic AMP induces the activity of the myelin enzyme, 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP; EC 3.1.4.37), in C6 glioma cells. Immunotitration experiments and measurements of the accumulation of [35S]methionine-labeled CNP showed that dibutyryl cyclic AMP increased the amount of CNP in the cells but not the catalytic activity per molecule of the enzyme. Moreover, inhibition of protein synthesis with cycloheximide abolished induction of enzyme activity. Dibutyryl cyclic AMP doubled the rate of CNP synthesis but had no effect on the half-life of the enzyme (∼33 h). The induction was partially blocked by the inhibitors of mRNA synthesis, cordycepin or α-amanitin. Thus, cyclic AMP induces the synthesis of CNP.Keywords
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