Typing forHLA-DPB1 * 03 andHLA-DPB1 * 06 using allele-specific DNA in vitro amplification and allele-specific oligonucleotide probes. Detection of “new” DPB1*06 variants
- 1 September 1989
- journal article
- research article
- Published by Springer Nature in Immunogenetics
- Vol. 30 (3) , 208-213
- https://doi.org/10.1007/bf02421208
Abstract
DP gene typing using in vitro DNA amplification combined with sequence-specific oligonucleotide probes has recently been reported. The resulting DNA amplification was specific for theHLA-DPB locus. Typing for the individualDPB alleles was exclusively dependent on the hybridizations of the probes but hampered by close sequence homology between differentDP alleles yielding complex patterns of reactivity with a panel of probes. We report the combined use of allele-specific DNA in vitro amplification and allele-specific oligonucleotides in typing forDPB1 * 03 andDPB1 * 06. Complete concordance with PLT typing was observed for theDPB1 * 03 alleles, while in the DPB1*06 group, at least three variantDPB1 * 06 alleles were identified which have not been described previously.Keywords
This publication has 19 references indexed in Scilit:
- DNA-RFLP analysis and genotyping of HLA-DR and DQ antigensImmunology Today, 1988
- Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.Proceedings of the National Academy of Sciences, 1988
- Allelic variation in the DR subregion of the human major histocompatibility complex.Proceedings of the National Academy of Sciences, 1987
- Identification of HLA-DP polymorphism with DP alpha and DP beta probes and monoclonal antibodies: correlation with primed lymphocyte typing.Proceedings of the National Academy of Sciences, 1987
- Restriction fragment length polymorphism of the HLA-DP subregion and correlations to HLA-DP phenotypes.Proceedings of the National Academy of Sciences, 1987
- Exon-specific oligonucleotide probes localize HLA-DQ ? allelic polymorphismsImmunogenetics, 1986
- Interaction between a "processed" ovalbumin peptide and Ia molecules.Proceedings of the National Academy of Sciences, 1986
- Binding of immunogenic peptides to Ia histocompatibility moleculesNature, 1985
- DNA Polymorphism of HLA Class I and Class II RegionsImmunological Reviews, 1985
- Human class II major histocompatibility antigen β-chains are derived from at least three lociNature, 1983