Simple Procedures for Enrichment of Chlorinated Aromatic Pollutants from Fat, Water and Milk for Subsequent Analysis by High-Resolution Methods

Publisher Website
Google Scholar
Abstract
Procedures for enrichment of nonvolatile chlorinated aromatic pollutants from fat, water and milk were described. 14C-DDT was used as a model compound in recovery experiments. A several thousand-fold enrichment of DDT added to butter was achieved by 2 consecutive straight-phase chromatographies on Lipidex 5000. Trace amounts of DDT in liter volumes of water were quantitatively extracted by rapid filtration through 2-ml beds of Lipidex 1000. A batch extraction procedure permitted enrichment of DDT from milk after addition of n-pentylamine, methanol and water. DDT was eluted from the gel with retention of > 90% of the lipids. A reversed-phase system with Lipidex 5000 was used for separation of TCDD [2,3,7,8-tetrachlorodibenzo-p-dioxin] from DDT and PCB [polychlorinated biphenyl]. The liquid-gel chromatographic procedures were simple and suitable for clean-up of samples prior to application of high-resolution methods.