Suppression of 3-methylcholanthrene-induced celluar tranformation by timed administration of the Bowman-Birk protease inhibitor

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Abstract
The Bowman-Birk protease inhibitor (BBI) is a legumederived inhibitor of chmotrypsin and trypsin that has been shown to suppress cellular transformation and tumorigenesis. In the present investigation the effects of various BBI adminsitration schedules were evalated for suppression of 3-methylchoilanthrene (3-MCA)-induced transormation of C3H/101T1/2 cells. At a concentration of 30 μg/ml, BBi demonstrated no toxicity to C3H/10T1/2 cells treated with 3-MCA. However, transformation of C3H/10T1/2 cells was significantly reduced with BBI was added to the cultures for a period of 14 days, starting immdediately after exposure to the carcinogen. When BBI was administered only during the time of carcinogen exposure or alternatively beginning on day 15 and then continuously throughtou the remainder of the 6-week transformation assay, it was ineffective for suppressing 3-MCA-induced cellular transformaiton. These findings indicate that BBI exerts its chemopreventive effect during a early stage of chemicalcarcinogen-induced cellular transformation.

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