An Analysis of Seed Development inPisum sativum

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Abstract
The expression of storage protein genes has been followed during the normal development of pea (Pisum sativum L.) embryos and compared with that in cultured embryos using complementary DNAs (cDNAs) for the major storage protein classes, vicilin, legumin and convicilin. The maximum level of vicilin mRNA in vivo was achieved earlier than that of legumin and convicilin. All three classes of storage protein mRNA were detected in cultured embryos but their amounts were altered, on a temporal basis, from those observed for embryos developing on the plant. The amounts of mRNAs per unit fresh weight produced in cultured embryos of less than 125 mg fresh weight, however, equalled or exceeded those of embryos from the plant. It is suggested that the effects observed in culture are linked with a shift in the cotyledonary cell population from cell division to cell expansion and the premature ‘ageing’ of the embryo.

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