Pyridine nucleotide transhydrogenase in normal human and leukemic leukocytes.

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Abstract
The enzyme TPNH-DPN or TD reduced [triphosphopyridine nucleotide-dlphosphopyridine nucleotide] transhydrogenase activity was assayed in the supernatant and particulate fraction of the leukocytes from 24 normal subjects and 47 patients with leukemia. The cells were prepared by Dextran sedimentation and red cells were lysed by hypotonic saline. The frozen packed white cells were subjected to sonic oscillation for 2 min. and centrifuged at 100,00 g for 1 hr. The results were calculated as millimicromoles of AcPyDPN reduced/hr./mg of protein. Little TD activity was found in the supernatant fraction. The enzyme activity in the sediment of cells from patients with chronic lymphocytic, acute myeloid, and acute lymphocytic leukemia was significantly higher than that in the cells from normal controls. The highest activity was found in the cells of acute lymphocytic leukemia and it was significantly higher than that found in the cells from acute myeloid leukemia.
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