5′-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II
Open Access
- 15 December 1997
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 11 (24) , 3306-3318
- https://doi.org/10.1101/gad.11.24.3306
Abstract
We have investigated the role of the RNA Polymerase II (Pol II) carboxy-terminal domain (CTD) in mRNA 5′ capping. Transcripts made in vivo by Pol II with a truncated CTD had a lower proportion of capped 5′ ends than those made by Pol II with a full-length CTD. In addition, the enzymes responsible for cap synthesis, RNA guanylyltransferase, and RNA (guanine-7)-methyltransferase bound directly to the phosphorylated, but not to the nonphosphorylated, form of the CTD in vitro. These results suggest that: (1) Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and (2) capping is co-ordinated with CTD phosphorylation.Keywords
This publication has 58 references indexed in Scilit:
- RNA capping enzyme and DNA ligase: a superfamily of covalent nucleotidyl transferasesMolecular Microbiology, 1995
- RNA polymerase II C-terminal domain required for enhancer-driven transcriptionNature, 1995
- Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongationNature, 1994
- In vivo transcriptional pausing and cap formation on three Drosophila heat shock genes.Proceedings of the National Academy of Sciences, 1993
- Positive patches and negative noodles: linking RNA processing to transcription?Trends in Biochemical Sciences, 1993
- DNA binding provides a signal for phosphorylation of the RNA polymerase II heptapeptide repeats.Genes & Development, 1992
- Multiple factors are required for specific RNA cleavage at a poly(A) addition site.Genes & Development, 1988
- Simian virus 40 enhancer increases number of RNA polymerase II molecules on linked DNANature, 1985
- mRNA cap binding proteins: essential factors for initiating translationCell, 1985
- The addition of 5′ cap structures occurs early in hnRNA synthesis and prematurely terminated molecules are cappedCell, 1980