Effects of Nutritional Factors on Formation of Scopoletin and Scopolin in Tobacco Tissue Cultures

Abstract
At low concentrations, sucrose and phosphate increased the formation of scopoletin and scopolin. Among carbon sources tested, only sucrose was effective in the formation of scopoletin and scopolin. A high concentration of either inorganic nitrogen source or casamino acid increased scopoletin and scopolin formation. l-Phenylalanine as the sole amino acid completely inhibited cell growth and the formation of scopoletin and scopolin, but combining l-phenylalanine and casamino acid remarkably stimulated the formation of scopoletin and scopolin.