Synthesis and Analytical Use of 3-Carboxypropionyl-alanyl-alanyl-valine-4-nitroanilide: A Specific Substrate for Human Leukocyte Elastase
- 31 December 1979
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 361 (2) , 1413-1416
- https://doi.org/10.1515/bchm2.1980.361.2.1413
Abstract
A simple synthesis is described for 3-carboxypropionyl-Ala-Ala-Val-4-nitroanilide, a convenient and very specific substrate for human leukocyte elastase (Km = 1.0 mM, kcat = 8.7 s-1). The substrate does not undergo appreciable spontaneous hydrolysis. It is not cleaved by trypsin or chymotrypsin but is cleaved slowly by porcine pancreatic elastase (Km = 9.1 mM, kcat = 1.4 s-1).This publication has 8 references indexed in Scilit:
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