Simultaneous and Accurate Determination of One-Bond 15N−13C‘ and Two-Bond 1HN−13C‘ Dipolar Couplings
- 29 August 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 125 (38) , 11504-11505
- https://doi.org/10.1021/ja035954e
Abstract
Using the echo−anti-echo manipulation, the 15N− 1HN cross-peaks split in the E.COSY spectrum by the 13CO couplings are separated into different, distinct regions in the HSQC spectrum. From this novel E.COSY 15N−1HN HSQC spectrum, the small one-bond 15N−13C‘ and two-bond 1HN−13C‘ residual dipolar couplings can be extracted easily and accurately. These dipolar couplings provide a set of important long-range constraints for protein structure determination.Keywords
This publication has 12 references indexed in Scilit:
- Sensitivity-enhanced 2D IPAP, TROSY–anti-TROSY, and E.COSY experiments: alternatives for measuring dipolar couplingsJournal of Magnetic Resonance, 2003
- Sensitivity-enhanced E.COSY-type HSQC experiments for accurate measurements of one-bond 15N–
H N and 15N–C C H N residual dipolar couplings in proteinsJournal of Magnetic Resonance, 2002 - Prediction of Sterically Induced Alignment in a Dilute Liquid Crystalline Phase: Aid to Protein Structure Determination by NMRJournal of the American Chemical Society, 2000
- Improving the Packing and Accuracy of NMR Structures with a Pseudopotential for the Radius of GyrationJournal of the American Chemical Society, 1999
- Determination of Relative N−HN, N−C‘, Cα−C‘, and Cα−Hα Effective Bond Lengths in a Protein by NMR in a Dilute Liquid Crystalline PhaseJournal of the American Chemical Society, 1998
- Measurement ofJand Dipolar Couplings from Simplified Two-Dimensional NMR SpectraJournal of Magnetic Resonance, 1998
- NMRPipe: A multidimensional spectral processing system based on UNIX pipesJournal of Biomolecular NMR, 1995
- Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution.Proceedings of the National Academy of Sciences, 1995
- Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutionsJournal of Biomolecular NMR, 1992
- A Novel, Highly Stable Fold of the Immunoglobulin Binding Domain of Streptococcal Protein GScience, 1991