In Vitro Responses of Adventitious Embryos of Two Polyembryonic Eugenia Species

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Abstract
Ovules were excised from immature fruit of 2 naturally polyembryonic, tropical trees, Eugenia jambos and E. malaccensis. Immature, adventitious embryos were removed from the ovules and were cultured on modified Murashige and Skoog (MS) medium that contained either 0-10 mg/liter benzylaminopurine (BA) or 0-10 mg/liter 2,4-dichlorophenoxyacetic acid (2,4-D). Depending on the developmental stage of the adventitious embryos, proliferation of axillary buds occurred on media with 2-10 mg/liter BA, whereas germination occurred on lower BA concentrations or in the absence of growth regulators. Root formation only was induced on media containing 3-10 mg/liter 2,4-D. Embryogenic callus was formed from adventitious embryos from 1-2 cm fruitlets on 1-2 mg/liter 2,4-D. The 2 species responded in a similar manner.

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