Separation and Ultrastructure of Proplastids from Dark-grown Euglena Cells
Open Access
- 1 June 1973
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 51 (6) , 1109-1116
- https://doi.org/10.1104/pp.51.6.1109
Abstract
A procedure for the separation of proplastids free of mitochondria from dark-grown Euglena cells has been developed. A fraction enriched in proplastids was used for freeze-etching study of proplastid structure. The prolamellar body in freeze-etched replicas appeared sponge-like, with thylakoids, often vesicular, emerging from it. The prolamellar body and the thylakoids were covered by particles of about 100Å in diameter. No larger particles, typical of light-grown chloroplasts, were observed.Keywords
This publication has 14 references indexed in Scilit:
- Structural Characteristics of a Photosynthetic Mutant of Euglena gracilis Blocked in Photosystem IIPlant Physiology, 1972
- Events surrounding the early development of Euglena chloroplastsDevelopmental Biology, 1972
- Chloroplast Structure and BiogenesisAnnual Review of Biochemistry, 1971
- Chloroplast ribosomes in the proplastids of Euglena gracilisBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Structure et evolution du corps prolamellaire dans les proplastes D'Euglena gracilisExperimental Cell Research, 1971
- A COMPARISON OF CHLOROPLAST MEMBRANE SURFACES VISUALIZED BY FREEZE-ETCH AND NEGATIVE STAINING TECHNIQUES; AND ULTRASTRUCTURAL CHARACTERIZATION OF MEMBRANE FRACTIONS OBTAINED FROM DIGITONIN-TREATED SPINACH CHLOROPLASTSThe Journal of cell biology, 1969
- Measurements of s-ϱ coordinates during the development of Euglena chloroplastsBiochimica et Biophysica Acta (BBA) - General Subjects, 1968
- Studies of Chloroplast Development in Euglena. VII. Fine Structure of the Developing PlastidPlant Physiology, 1964
- A NEW FREEZING-ULTRAMICROTOMEThe Journal of cell biology, 1961
- Fluorescence Studies of Chloroplast Development in EuglenaNature, 1960