DIFFERENCES IN THE PURIFICATION EFFECT OF CARCINOEMBRYONIC ANTIGEN (CEA) FROM THE 3 DIFFERENT HEPATIC METASTASES OF RECTUM CARCINOMA

  • 1 January 1979
    • journal article
    • research article
    • Vol. 26  (2) , 157-167
Abstract
Carcinoembryonic antigen (CEA) was purified from 3 [human] liver metastases of carcinoma recti by a conventional procedure involving perchloric acid extraction of tumor tissue and fractionation of the extract by gel filtrations on Sepharose 4B and Sephadex G-200. The preparations obtained showed a different degree of heterogeneity in SDS-polyacrylamide gel electrophoresis and crossed immunoelectrophoresis, and also a different antigenic activity. One of preparations obtained was a homogeneous CEA by all these criteria, and 2 others showed heterogeneity and lower antigenic activity. The most heterogeneous CEA preparation was further purified by the affinity chromatography on concanavalin A-Sepharose 4B and by DEAE-Sephadex A-50 chromatography and the usefulness of these procedures for purification of CEA was compared.