Fluorometric determination in biological fluids of the release kinetics of liposome-entrapped doxorubicin

Publisher Website
Google Scholar
Abstract
A fluorometric method is presented that allows a continuous monitoring in 50% (v/v) human serum of the release of liposome-entrapped doxorubicin (DXR).This method exploits the intrinsic fluorescence of DXR and uses DNA as a fluorescence quencher of the released drug. It is much more simple and rapid than the commonly used methods. It is shown that this method can be applied to the study of the stability of liposomal-DXR encapsulated using either an ammonium sulfate gradient or a pH gradient. Small unilamellar vesicles (SUVs) made from egg yolk phosphatidylcholines (EPC) were much less stable, either in phosphate buffer saline or in human serum, than SUVs made from a 1/1 mixture of EPC and cholesterol (Choi). SUVs made from EPC/Chol loaded with DXR using an ammonium sulfate gradient were found to be more stable than those loaded using a pH gradient.
Keywords

This publication has 11 references indexed in Scilit: