Postmortem Accumulation of Tubulin in Postsynaptic Density Preparations

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Abstract
Postsynaptic density (PSD) preparations isolated from canine cerebral cortex that were left at 0-37.degree. C for various times were enriched in 2 bands in a time- but not temperature-dependent manner. The 2 bands were identified as tubulin subunits by gel mobility and immunology. Of all the isolated synaptic structures the increase in tubulin occurred primarily in the PSD fraction. The increase of tubulin also occurred in PSD preparations isolated from canine cerebellum and rat forebrain. Results obtained when PSD fractions were isolated from canine brain obtained as rapidly as possible after the death of the animal indicate that the maximum amount of tubulin in the PSD preparations is 2.5% of total Coomassie blue-stained protein, as determined by scanning of gel electrophoretograms. Tubulin is probably not a major structural protein of the PSD as it exists in situ.