Human serum bilirubin fractionation in various hepatobiliary diseases by the newly developed high performance liquid chromatography

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Abstract
Serum Bilirubin was fractionated by newly developed reversed phase high performance liquid chromatography (HPLC) into 5 fractions: δ (δ-Bilirubin,Bδ),γ (bilirubin diglucuronide, BDG), β (Bilirubin monoglucuronide, BMG), β′ ((Z, E,) and/or (E, Z)-bilirubin IXα) and α ((Z, Z)-bilirubinIXα). Sera of healthy subjects and of patients with unconjugated hyperbilirubinemia showed predominantly a fraction with a small amount of β′ fraction. Trace amounts of δ fraction were detected in a few cases. The results of fractionation of serum bilirubin in 159 patients with various hepatobiliary diseases suggested that the ratios B<5/(Bδ+BDG+BMG) and BMG/Bδ can be useful parameters to follow patients with jaundice, compared with the reported Bδ/total bilirubin which did not always reflect the jaundice stage, especially in cases with low serum bilirubin levels.