Comparative Study of Isoenzyme Formation of Bacterial β-Galactosidase
Open Access
- 1 April 1970
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 102 (1) , 79-84
- https://doi.org/10.1128/jb.102.1.79-84.1970
Abstract
The enzyme β-galactosidase was studied in crude extracts of Escherichia coli 3300, E. coli grown on a selenium and sulfur medium, Salmonella typhimurium F-lac, Serratia marcescens F-lac, S. marcescens P-lac, Proteus mirabilis F-lac, P. mirabilis P-lac, Aeromonas formicans , and Streptococcus lactis . The isoenzymes could be demonstrated by an alternative histochemical technique. Different isoenzyme patterns were found to be determined by the β-galactosidase structural gene and not by the cytoplasm within which the β-galactosidase was formed. In addition, the β-galactosidases from strains which form isoenzymes were more stable to heat and urea treatments than the enzyme formed by those organisms which produce reduced amounts of, or no, isoenzyme.Keywords
This publication has 15 references indexed in Scilit:
- ISOZYMES OF THE LACTOSE OPERON OF ESCHERICHIA COLIAnnals of the New York Academy of Sciences, 1968
- Catabolite repression of the synthesis of β-galactosidase in Proteus mirabilis F-lacBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- The regulation of the synthesis of β-galactosidase in Proteus mirabilis F-lacBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- Purification and comparison of the β-galactosidase synthesized by Escherichia coli F-lac+ and Proteus mirabilis F-lac+Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- The isolation and properties of β-galactosidase from Escherichia coli grown on sodium selenateBiochimica et Biophysica Acta (BBA) - General Subjects, 1967
- Growth of Escherichia coli on selenateBiochimica et Biophysica Acta (BBA) - General Subjects, 1967
- Purification, Composition, and Molecular Weight of the β-Galactosidase of Escherichia coli K12Published by Elsevier ,1965
- β-Galactosidase of Streptococcus lactisJournal of Bacteriology, 1965
- Multiple molecular forms of β-galactosidaseJournal of Molecular Biology, 1965
- TRANSFER OF EPISOMIC ELEMENTS TO PROTEUS IJournal of Bacteriology, 1964