Detection and Subtyping of Foot-and-Mouth Disease Virus in Infected Cattle by Polymerase chain Reaction and Amplified VP1 Sequencing
Open Access
- 1 January 1995
- journal article
- research article
- Published by SAGE Publications in Journal of Veterinary Diagnostic Investigation
- Vol. 7 (1) , 52-55
- https://doi.org/10.1177/104063879500700107
Abstract
Fast and accurate detection of foot-and-mouth disease (FMD) outbreaks is needed to limit spread of the disease by proper vaccination. The use of the polymerase chain reaction (PCR) has revolutionized the way in which viral diseases are diagnosed. Sequence analysis of the amplified VP1 sequence can enable the classification of FMD virus detected in the morbid animal. PCR assays were carried out to identify the virus and its serotype in suspect animals from 2 outbreaks of FMD type O virus. Sequence analysis of the amplified VP1 cDNA showed 78% homology with O1K and over 95% homology between the samples. These findings suggest that the 2 outbreaks were due to infection with the same virus serosubtype.Keywords
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