Identification of expressed genes by laser-mediated manipulation of single cells
- 1 August 1998
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 16 (8) , 737-742
- https://doi.org/10.1038/nbt0898-737
Abstract
We describe a rapid noncontact method for the capture of single cells or small tissue areas of any size or shape directly within the cap of a common microfuge tube. Prior to the laser-mediated transfer, the specimen is isolated by laser microbeam microdissection, forming a clear-cut gap around the selected area. Laser treatment does not impair subsequent RNA analysis. We have used this method to isolate a single cell from archival colon adenocarcinoma, and were able to detect point mutations within codon 12 of c-Ki-ras2 mRNA after nested RT-PCR analysis.Keywords
This publication has 29 references indexed in Scilit:
- Laser beam microdissection in the diagnosis of cutaneous B-cell lymphomaBritish Journal of Dermatology, 1997
- Laser beam microdissection in the diagnosis of cutaneous B-cell lymphomaBritish Journal of Dermatology, 1997
- Cut out or poke in—the key to the world of single genes: laser micromanipulation as a valuable tool on the look-out for the origin of diseaseGenetic Analysis: Biomolecular Engineering, 1997
- Laser microdissection of metaphase chromosomes and characterization by atomic force microscopyJournal of Biomedical Optics, 1997
- Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezersJournal of Assisted Reproduction and Genetics, 1996
- Wavelength dependence of laser-induced DNA damage in lymphocytes observed by single-cell gel electrophoresisJournal of Photochemistry and Photobiology B: Biology, 1995
- A simple, rapid method for isolating RNA from paraffin-embedded tissues for reverse transcription-polymerase chain reaction (RT-PCR).Journal of Histochemistry & Cytochemistry, 1994
- Genetic Alterations during Colorectal-Tumor DevelopmentNew England Journal of Medicine, 1988
- ras GENESAnnual Review of Biochemistry, 1987
- Ras GenesAnnual Review of Biochemistry, 1987