Effects of heat shock on the level of trehalose and glycogen, and on the induction of thermotolerance in Neurospora crassa

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Abstract
Neurospora crassa conidiospore germlings exposed to a heat shock (30–45°C) rapidly accumulated trehalose and degraded glycogen, even in the presence of cycloheximide. This phenomenon was also rapidly reversible upon return of the cells at 30°C. Trehalose accumulation at 45°C demanded an exogenous source of carbon and either glucose or glycerol fulfilled such requirement. Experiments with the cyclic AMP-deficient cr-1 mutant suggested that the effects of temperature shifts on trehalose level were independent of cAMP metabolism. Cells exposed at 45°C under conditions permissive for trehalose accumulation (i.e. in the presence of an assimilable carbon source) also acquired thermotolerance.