Classification of Mycobacterium farcinogenes and Mycobacterium senegalense by Immunodiffusion and Thin-layer Chromatography of Long-chain Components

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Abstract
Comparative immunodiffusion studies and TLC analyses of whole-organism acid methanolysates were performed on 37 strains of M. farcinogenes, M. senegalense and Nocardia farcinica. The latter were clearly distinguished from the mycobacteria in containing a single mycolic acid methyl ester and showing more precipitinogens with nocardial than with mycobacterial and rhodococcal reference systems. The distribution of precipitinogens showed that M. farcinogenes and M. senegalense were very closely related and that both showed a greater affinity to M. fortuitum than to any of the other established Mycobacterium spp. tested. The complex pattern of .alpha.-mycolates and characteristic polar mycolates found in M. farcinogenes and M. senegalense was found previously in M. fortuitum and M. smegmatis.