NON-RANDOM NUCLEAR ORIENTATION DURING DNA SYNTHESIS IN TRADESCANTIA POLLEN GRAINS

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Abstract
When Tradescantia pollen grains are isolated at stages shortly after the micros pore mitosis and incubated with radioactive thymidine, autoradiographs show that the DNA of the generative nucleus is labeled. The vegetative nucleus is displaced from its usual position in the middle of the vegetative cell, and moves toward either end of the cell. The movement is measurable in fixed, Feulgen- stained pollen grains as an angular displacement from the short axis of the pollen grain. Labeled pollen grains show a larger mean displacement (skewness) than unlabeled ones. This correlation is discussed with respect to the possible interrelation between the metabolism of the 2 cells in the pollen grain.

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