Efficient, direct plant regeneration from stem segments of chrysanthemum (Chrysanthemum morifolium Ramat. cv. Royal Purple)

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Abstract
Direct plant regeneration was obtained from fresh chrysanthemum (Chrysanthemum morifolium Ramat. cv. Royal Purple) stem segments cultured on Murashige and Skoog's (1962) basal media supplemented with 6-benzylaminopurine (BAP, 0.5–2.0 mg/l) and α-naphthaleneacetic acid (NAA, 0.2–2.0 mg/l). The morphogenetic potential varied with the developmental stage of the stem explant. The highest percentage of shoot formation (100%) and greatest average number of shoots per explant (14.6) were observed on stem segments taken from the top of the cutting. This organogenetic capacity decreases in the more mature stem. Normal, flowering plants were obtained three to four months after culture.