PHYSIOLOGICAL FACTORS OPERATIVE IN ICE-SHEET INJURY OF ALFALFA

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Abstract
A series of laboratory investigations were conducted to study the highly injurious character of prolonged and contacting sheets of ice to overwintering field crops. Cold-hardened plants of alfalfa were stored at harmless but low ( + 1[degree] and [long dash]4[degree]C) temps. in various gaseovis, liquid, and solid (frozen in ice) media. Conditions of storage, such as in ice, stoppered tubes of water, CO2, N2, and air, appeared to inhibit removal of the products of anaerobic and aerobic respiration until the accumulations became toxic and highly lethal. Storage for long periods in such media as flowing air and water through which air was bubbled was not injurious. The ice itself was not directly harmful. Injury occurred only after 7-10 days of storage in this medium. CO2 was the only one of the several storage media which appeared to be directly toxic and it was particularly so when in soln.

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