Three‐dimensional structure of the oligosaccharide terminus of globotriaosylceramide and isoglobotriaosylceramide in solution

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Abstract
Spatial structures of the oligosaccharide parts of globotriaosylceramide, Gal(.alpha.1-4)Gal(.beta.1-4)Glc(.beta.1-1)Cer (Cer = ceramide) and isoglobotriaosylceramide, Gal(.alpha.1-3)Gal(.beta.1-4)Glc(.beta.1-1)Cer were investigated in (C2H3)2SO solution by means of labortory and rotating frame NOE, hydroxyl protons being used as long-range sensors defining the distance constraints. Both oligosaccharides were found to exist in more than on conformation interconverting rapidly one the NMR time scale. The conformation of the Gal(.alpha.1-4)Gal(.beta.1-4)Glc.beta. trisaccharide dissolved in 2H2O appeared to be the same as that of the corresponding part of the glycosphingolipid in (C2H3)2SO solution.