A New Method for the Preparation of Acyl-CoA Thioesters1
- 1 February 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 89 (2) , 337-339
- https://doi.org/10.1093/oxfordjournals.jbchem.a133207
Abstract
A method is described for the preparation of CoA thioesters of fatty acids. Acyl-CoA thioester was synthesized by way of 1-acylimidazole and purified by high performance liquid chromatography. This method is applicable to the preparation of a variety of acyl-CoA thioesters of various chain lengths (from C 8 to C 18 ) and with varying degrees of unsaturation. It is particularly suitable for preparing acyl-CoA thioesters of labeled fatty acids or fatty acids available only in small amounts.Keywords
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