Plaque formation by African swine fever virus in chick embryo fibroblasts in the absence of CO2 atmosphere
- 1 September 1976
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 52 (3) , 207-216
- https://doi.org/10.1007/bf01348018
Abstract
A plaque assay method using ASFV previously adapted to growth in chick embryo fibroblasts is described. Chick embryo fibroblast monolayers under bactoagar or methylcellulose have been employed using cysteine, arginine, DEAE-Dextran and HEPES as additives. Plaque production was optimal under methylcellulose. HEPES rendered the plaques more clear when used with the overlay. Arginine enhances plaque formation with bactoagar, and DEAE-Dextran doubles the plaque size. The growth curve of ASFV in chick embryo monolayers has been studied.Keywords
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