Retrograde Axonal Transport of Endogenous Proteins in Sciatic Nerve Demonstrated by Covalent Labeling in Vivo
- 18 April 1980
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 208 (4441) , 303-305
- https://doi.org/10.1126/science.6154312
Abstract
Extracellularly applied N-succinimidyl [2,3-3H]propionate was used in vivo to covalently label intra-axonal proteins in the rat sciatic nerve. This technique permitted a unique view of axonal transport of proteins independent of biosynthesis. The proteins detected in slow anterograde transport (1 to 2 millimeters per day) correspond to cytoskeletal proteins described in previous papers. The slowly retrogradely transported component (3 to 6 millimeters per day) was composed primarily of a single protein with a molecular weight of 68,000.Keywords
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