PROSTATIC NUCLEAR CHROMATIN: AN EFFECT OF TESTOSTERONE ON THE SYNTHESIS OF RIBONUCLEIC ACID RICH IN CYTIDYLYL(3′,5′)GUANOSINE

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Abstract
Prostatic nuclear chromatin [rat] can be separated into 4 categories according to its capacity for the synthesis in vitro of different species of RNA by DNA-dependent RNA polymerases. M regions occupy about 70-80% of the total nuclear DNA, and DNA at this region is not accessible to actinomycin D or RNA polymerase. R regions occupy about 20-30% of total nuclear DNA. This DNA is available for the binding of actinomycin and can function as template for RNA synthesis by additional bacterial polymerase when nucleus is ruptured. Ch regions have about 1% of total nuclear DNA. RNA synthesis at this region of intact nucleus continues even after animals are deprived of androgens. Only a very small portion (1% or less) of nuclear DNA is associated with No regions where androgen in vivo provokes selective enhancement of RNA synthesis. Nearest neighbor frequency study suggested that the nucleotide sequence in the DNA of each region is unique. The deoxycytidylyl (3[image],5[image])deoxyguanosine sequence in the prostatic DNA appears to have a strinking nonrandom distribution. The apparent content of this base sequence in DAN of No regions was about 24, 8, and 2 times, respectively, of that in DNA of M, R, and Ch regions.