Cholesterol induced lipid accumulation in myocardial cells of rats
- 1 March 1993
- journal article
- research article
- Published by Oxford University Press (OUP) in Cardiovascular Research
- Vol. 27 (3) , 442-446
- https://doi.org/10.1093/cvr/27.3.442
Abstract
Objective: The aim was to investigate whether cholesterol feeding of rats for only 10 d would result in lipid accumulation in myocardial cells and a change in the peroxisomal β oxidation in the heart compared to the hearts of rats on standard feeds. Methods: Eight rats received a cholesterol diet (2%) and eight rats received a standard diet for 10 d. Lipids were measured in serum, liver, and heart. Palmitoyl-CoA hydrolase and fatty acyl-CoA oxidase were determined in total homogenate of hearts. The fractional volume of lipid droplets in myocardial cells was calculated from micrographs at a magnification of ×9600 obtained by electron microscopy. Results: The fractional volume of lipid droplets in the cardiomyocytes increased from 0.109(SEM 0.019)% to 0.259(0.037)%, (p−1 wet weight compared to 5.87(0.93) μmol·g−1 in the controls on standard diet (p−1 wet weight to 53.3(6.3) μmol·g−1. Conclusions: Lipid accumulation in the myocardial cells was shown by morphometry after 10 d of cholesterol feeding, without any visible damage to the tissue. Cardiovascular Research 1993;27:442-446Keywords
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