Behavioral effects of prenatal folate deficiency in mice
- 2 March 2005
- journal article
- research article
- Published by Wiley in Birth Defects Research Part A: Clinical and Molecular Teratology
- Vol. 73 (4) , 249-252
- https://doi.org/10.1002/bdra.20111
Abstract
BACKGROUND Folate supplementation decreases the incidence of birth defects such as neural tube defects (NTDs). We and others have shown that gestational dietary folate deficiency that does not produce overt NTDs can alter fetal neural histology. Accordingly, murine offspring were examined for the possible functional consequences of prenatal folate deficiency. METHODS CD-1 mice were fed a diet of chow containing 400, 600, or 1200 nmol of folic acid/kg of chow for eight weeks prior to breeding and until GD18, at which time all dams were placed on folate-replete chow. Behavioral tests of male and female offspring included righting reflex, negative geotaxis, forelimb hanging, motor coordination, open field activity, and elevated plus maze activity. RESULTS Of greatest significance, the adult offspring that were prenatally folate-deficient exhibited more anxiety-related behavior in the elevated plus maze. Offspring of the 400 nmol of folic acid/kg of chow diet group exhibited significantly shorter durations in the open arms and longer durations in the closed arms. Further, these two behaviors were dose-related. There was also a trend for the prenatally folate-deficient adult mice to exhibit more thigmotaxis (wall-hugging) behavior in the open field, entering the central area less frequently than controls. There were few other differences in tested behaviors between folate-deficient and folate-replete mice. CONCLUSIONS Prenatal folate deficiency that is repleted at birth can manifest later with increased anxiety 9–12 weeks after birth. Birth Defects Research (Part A), 2005.Keywords
This publication has 21 references indexed in Scilit:
- Low Serum Folate Levels as a Risk Factor for Depressive Mood in Patients With Chronic EpilepsyThe Journal of Neuropsychiatry and Clinical Neurosciences, 2003
- Generalized anxiety disorder: comorbidity, comparative biology and treatmentInternational Journal of Neuropsychopharmacology, 2002
- Investigation of the effects of folate deficiency on embryonic development through the establishment of a folate deficient mouse modelTeratology, 2002
- Homocysteine, folate, methylation, and monoamine metabolism in depressionJournal of Neurology, Neurosurgery & Psychiatry, 2000
- Hypothesis: Folate-responsive neural tube defects and neurocristopathiesTeratology, 2000
- Minimal behavioral effects from developmental cerebellar stunting in young rats induced by postnatal treatment with α-difluoromethylornithineNeurotoxicology and Teratology, 2000
- Neuroanatomical and functional alterations resulting from early postnatal cerebellar insults in rodentsPharmacology Biochemistry and Behavior, 1996
- Fertility in mice after prenatal exposure to benzo[a]pyrene and inorganic lead.Environmental Health Perspectives, 1995
- Age-related differences in behavior across the life span of the C57BL/6J mouseExperimental Aging Research, 1981
- Neurochemical and behavioral effects of diet related perinatal folic acid restrictionPharmacology Biochemistry and Behavior, 1976