THE EFFECT OF COLUMN GEOMETRY ON SEPARATION EFFECTIVENESS OF AGAROSE FOR POLY(VINYL PYRROLIDONE)

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Abstract
Glass columns packed with agarose gets were evaluated for the analytical fractionation of poly(vinyl pyrrolidone). Columns were studied with inside diameters ranging from 0.56 to 1.58 cm. and lengths ranging from 28 to 120 cm. Column efficiencies were found to vary from 250 plates per foot for the least effective column to 1600 plates per foot for a 1.10 by 44 cm. column. The efficiency was judged by the usual criterion of band spreading for a low molecular weight material, in this case 2-pyrrolidone. Another measure of effectiveness used was the separation obtained between two fractions of poly(vinyl pyrrolidone) with molecular weights of 12,700 and 58,500. The column geometry giving the highest plate count also separated the polymer fractions most effectively.