PROTEASES OCCURRING IN THE CELL-MEMBRANE - A POSSIBLE CELL-RECEPTOR FOR THE BOWMAN-BIRK TYPE OF PROTEASE INHIBITORS .1.
- 15 March 1987
- journal article
- research article
- Vol. 47 (6) , 1598-1601
Abstract
The legume-derived Bowman-Birk trypsin and chymotrypsin protease inhibitors (BBI) are effective anticarcinogens in vivo and in vitro. The chymotrypsin-inhibitory domain has been shown to be responsible for this anticarcinogenic action. In this study, we identify hydrolytic enzymes by their ability to hydrolyze the relatively specific chymotrypsin substrate succinyl-Ala-Ala-Pro-Phe-aminomethyl coumarin. Results presented in this study show: (a) there is an approximately 2-fold increase in the activity of these enzyme(s) between normal and transformed C3H/10T 1/2 cells; (b) there are five such enzymes associated with transformed cells (separated by diethylaminoethyl-cellulose chromatography); (c) only two of these enzymes are inhibited by BBI; (d) the BBI-inhibitable enzymes are membrane associated; (e) the BBI-inhibitable enzymes are similar to each other but different from pancreatic chymotrypsin. BBI has thus distinguished a subpopulation of enzymes capable of hydrolyzing succinyl-Ala-Ala-Pro-Phe-aminomethyl coumarin which may mediate the transformation of C3H/10T 1/2 cells.This publication has 12 references indexed in Scilit:
- Inhibition of fibrinogen receptor expression and serotonin release by leupeptin and antipain.Journal of Biological Chemistry, 1985
- Nanomolar concentrations of Bowman-Birk soybean protease inhibitor suppress x-ray-induced transformation in vitro.Proceedings of the National Academy of Sciences, 1985
- Mammalian chymotrypsin-like enzymes. Comparative reactivities of rat mast cell proteases, human and dog skin chymases, and human cathepsin G with peptide 4-nitroanilide substrates and with peptide chloromethyl ketone and sulfonyl fluoride inhibitorsBiochemistry, 1985
- Protease inhibitors reduce the frequency of spontaneous chromosome abnormalities in cells from patients with Bloom syndrome.Proceedings of the National Academy of Sciences, 1984
- Inhibition of plasminogen activator release from transformed chicken fibroblasts by a protease inhibitorCell, 1981
- EFFECTS OF PROTEASE INHIBITORS ON RADIATION TRANSFORMATION INVITRO1981
- Antipain and radiation effects on oncogenic transformation and sister chromatid exchanges in Syrian hamster embryo and mouse C3H/10T1/2 cellsCarcinogenesis: Integrative Cancer Research, 1981
- Association of a protease (plasminogen activator) with a specific membrane fraction isolated from transformed cells.The Journal of cell biology, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A pure trypsin inhibitor from soya beansBiochemical Journal, 1963