NEW TECHNIQUE FOR THE CYTOCHEMICAL EXAMINATION OF HUMAN HEMATOPOIETIC-CELLS GROWN IN AGAR-GEL

  • 1 January 1980
    • journal article
    • research article
    • Vol. 8  (3) , 339-344
Abstract
All colonies in the agar gel in a plastic dish can be observed as a whole plate preparation after being stained cytochemically. Granulocytes and monocytes/macrophages in a cluster or a colony were easily distinguished from each other by easterase activity in response to naphthol AS-D chloroacetate or .alpha.-naphthyl acetate. To illustrate this technique''s possiblities, a time course study on the formation of granulocyte and monocyte/macrophage clusters or colonies from normal human marrow cells was performed.

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