Nitric oxide‐dependent vasodilatation of rabbit femoral artery by β2‐adrenergic stimulation or cyclic AMP elevation in vivo
- 1 March 2000
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 129 (5) , 969-974
- https://doi.org/10.1038/sj.bjp.0703155
Abstract
Some studies suggest that β‐adrenoceptor‐mediated vasorelaxation is in part mediated through nitric oxide (NO) release. We wished to determine the contribution of the L‐arginine / NO system to vasodilatation in response to β‐adrenoceptor stimulation with isoprenaline or cyclic adenosine‐3′,5′‐monophosphate (cyclic AMP) elevation with forskolin and dibutyryl cyclic AMP in vivo, using a rabbit femoral artery constant perfusion model. Baseline femoral artery pressure was similar in rabbits receiving isoprenaline, forskolin or dibutyryl cyclic AMP. Isoprenaline, forskolin and dibutyryl cyclic AMP each decreased femoral artery pressure in a dose‐dependent manner. The doses (mol kg−1) of isoprenaline, forskolin and dibutyryl cyclic AMP which decreased pressure by 10% from baseline, expressed as a negative logarithm (−log ED10) were: 10.0±0.2, 9.5±0.1 and 4.9±0.1 respectively (P2‐adrenoceptor subtype. Injection of 1 μmol kg−1 NG‐nitro‐L‐arginine methyl ester (L‐NAME) did not alter baseline pressure. However, it abolished the pressure response to isoprenaline (P10 values for forskolin and dibutyryl cyclic AMP, in the presence of L‐NAME, were 7.9±0.1 and 3.5±0.3 respectively (P2‐adrenergic stimulation and cylic AMP elevation activate the L‐arginine/NO system in rabbit femoral artery in vivo, and that NO generation contributes importantly to the changes in vascular tone induced by agents which modulate β‐adrenoceptors or cyclic AMP. British Journal of Pharmacology (2000) 129, 969–974; doi:10.1038/sj.bjp.0703155Keywords
This publication has 31 references indexed in Scilit:
- Activation of nitric oxide synthase by β2‐adrenoceptors in human umbilical vein endothelium in vitroBritish Journal of Pharmacology, 1999
- Noradrenaline, β‐adrenoceptor mediated vasorelaxation and nitric oxide in large and small pulmonary arteries of the ratBritish Journal of Pharmacology, 1997
- Phorbol ester enhances activation of adenylate cyclase in Bovine aortic endothelial cellsLife Sciences, 1994
- Effect of NG-monomethyl-L-arginine on the β-adrenoceptor-mediated relaxation of rat mesenteric resistance arteriesLife Sciences, 1993
- The L-Arginine-Nitric Oxide PathwayNew England Journal of Medicine, 1993
- Flow activates an endothelial potassium channel to release an endogenous nitrovasodilator.Journal of Clinical Investigation, 1991
- β‐ADRENOCEPTORS ON ENDOTHELIAL CELLS DO NOT INFLUENCE RELEASE OF RELAXING FACTOR IN DOG CORONARY ARTERIESClinical and Experimental Pharmacology and Physiology, 1987
- AUTORADIOGRAPHIC LOCALIZATION OF RECEPTORS IN THE MAMMALIAN CARDIOVASCULAR SYSTEMClinical and Experimental Pharmacology and Physiology, 1987
- Endothelium-Removal Decreases Relaxations of Canine Coronary Arteries Caused by β-Adrenergic Agonists and AdenosineJournal of Cardiovascular Pharmacology, 1985
- The Pharmacology of a β2-Selective Adrenoceptor Antagonist (ICI 118,551)Journal of Cardiovascular Pharmacology, 1983