Optimal Conditions for the Estimation of Ammonium by the Berthelot Reaction

1 January 1978

journal article
research article
Published by SAGE Publications in Annals of Clinical Biochemistry: International Journal of Laboratory Medicine

Vol. 15 (1-6) , 270-275
https://doi.org/10.1177/000456327801500164

Abstract

A technique for optimising reagent concentrations on the AutoAnalyzer has been applied to the estimation of ammonia by the Berthelot reaction in the determination of urea and organic nitrogen. Comparison of the use of phenol and salicylate revealed that the optimum concentration of the latter is about four times that of the former. The optimum concentration of hypochlorite is five times greater with salicylate than with phenol, and for the catalyst, sodium nitroprusside, the factor is two. The precision obtained with the different methods is similar.

This publication has 10 references indexed in Scilit:

Tenofovir versus Placebo to Prevent Perinatal Transmission of Hepatitis B
New England Journal of Medicine, 2018
Photometric determination of nitrogen. Wet incineration followed by formation of indophenol blue with salicylate/hypochlorite
Clinica Chimica Acta; International Journal of Clinical Chemistry, 1976
Micro-Determination of Nitrogen
Critical Reviews in Analytical Chemistry, 1974
A New Time-Saving Method for Determining Nitrogen in Biologic Material
Clinical Chemistry, 1968
The Determination of Organic Nitrogen
Proceedings of the Association of Clinical Biochemists, 1967
Report on Small Hospital Laboratories
New England Journal of Medicine, 1967
An improved method for the estimation of ammonia in blood plasma
Clinica Chimica Acta; International Journal of Clinical Chemistry, 1967
The estimation of ammonia using the indophenol blue reaction
Clinica Chimica Acta; International Journal of Clinical Chemistry, 1966
An absorptiometric method for determining ammonia in boiler feed-water
The Analyst, 1964
Spectrophotometric Determination of Nitrogen in Total Micro-Kjeldahl Digests. Application of the Phenol-Hypochlorite Reaction to Microgram Amounts of Ammonia in a Total Digest of Biological Material.
Analytical Chemistry, 1963