Is a room-temperature crossmatch necessary for the detection of ABO errors?

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Abstract
The detection of anti-A and anti-B isohemagglutinins by low-ionic-strength saline tests at 37.degree. C and by the indirect antiglobulin technique, without an immediate-spin or room-temperature phase, was studied. Using such a procedure, all but one of 2746 patient blood samples reacted in accordance with ABO type when tested against A2 and B red cells. However, the discrepant sample also was nonreactive when tested by immediate-spin technique against saline-suspended A2 red cells. Compatibility tests performed at 37.degree. C in low-ionic-strength saline apparently are as sensitive as immediate-spin tests with saline-suspended red cells for the detection of ABO errors. Performing serologic tests for unexpected alloantibodies and donor-recipient compatibility without an immediate-spin or room-temperature phase abbreviates pretransfusion testing and reduces the detection of clinically insignificant alloantibodies solely reactive at room temperature.

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