Generation of VV‐hemorphin‐7 from globin by peritoneal macrophages
- 11 March 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 382 (1-2) , 37-42
- https://doi.org/10.1016/0014-5793(96)00144-5
Abstract
Bovine globin has been incubated with mice peritoneal macrophages in order to study its hydrolysis by lysosomal enzymes, among which chiefly cathepsin D. Analysis of resulting peptides, by reversed-phase high-performance liquid chromatography (RP-HPLC), shown the release of a bioactive peptide, VV-hemorphin-7. When a carboxyl proteinase inhibitor such as pepstatin A was added, no hemorphin was generated. Our results clearly demonstrated that VV-hemorphin-7 generation was principally due to cathepsin D. This study allowed us to hypothetize a possible pathway for in vivo hemorphins appearance from globin catabolism by macrophages.Keywords
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