A Method for Preparing Bead Shaped Immobilized Enzyme
- 1 June 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 40 (6) , 1143-1149
- https://doi.org/10.1080/00021369.1976.10862178
Abstract
Monomer solution containing enzyme was frozen to a small bead shape by cool solvent and polymerized by 60Co .gamma.-ray with a dose of 125-850 Krad. Thus, bead shaped immobilized enzymes with various diameters (0.02-15 mm) were obtained. The bead had spongy structure and large surface area. Consequently, even large sized bead shaped immobilized enzyme showed high enzymic activity. Retained activity of bead shaped immobilized invertase [Candida utilis] was 16-73% depending on bead size. Bead shaped lipase [Rhizopus delemer] (1 mm diameter) showed 13.5-42.5% of retained activity depending on monomer combinations.This publication has 1 reference indexed in Scilit:
- Proteins coupled to polyacrylamide beads using glutaraldehydeBiochemical and Biophysical Research Communications, 1971