Adaptation of Coxsackie Virus Strain A-10 to Human Amnion Cells in Tissue Culture.

Abstract
A tissue culture adapted strain of Coxsackie virus A-10 has been obtained from primary human amnion cells inoculated with virus from a highly purified lot of suckling mouse grown material. The adapted virus (A-10TC) was neutralized by Coxsackie virus A-10 antiserum. It was no longer virulent in mice and caused gross cytopathic changes in primary human amnion cells indistinguishable from those changes caused by the polioviruses. Plaque assays of the virus were obtained on monolayer cultures of primary human amnion cells.