Osmotic Activation of the HOG MAPK Pathway via Ste11p MAPKKK: Scaffold Role of Pbs2p MAPKK
- 13 June 1997
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 276 (5319) , 1702-1705
- https://doi.org/10.1126/science.276.5319.1702
Abstract
Exposure of the yeast Saccharomyces cerevisiae to high extracellular osmolarity induces the Sln1p-Ypd1p-Ssk1p two-component osmosensor to activate a mitogen-activated protein (MAP) kinase cascade composed of the Ssk2p and Ssk22p MAP kinase kinase kinases (MAPKKKs), the Pbs2p MAPKK, and the Hog1p MAPK. A second osmosensor, Sho1p, also activated Pbs2p and Hog1p, but did so through the Ste11p MAPKKK. Although Ste11p also participates in the mating pheromone–responsive MAPK cascade, there was no detectable cross talk between these two pathways. The MAPKK Pbs2p bound to the Sho1p osmosensor, the MAPKKK Ste11p, and the MAPK Hog1p. Thus, Pbs2p may serve as a scaffold protein.Keywords
This publication has 24 references indexed in Scilit:
- Association of the Yeast Pheromone Response G Protein βγ Subunits with the Map Kinase Scaffold Ste5pScience, 1995
- MAP kinase pathways in yeast: For mating and moreCell, 1995
- Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiaeCell, 1994
- Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiaeGene, 1994
- A two-component system that regulates an osmosensing MAP kinase cascade in yeastNature, 1994
- The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5.Genes & Development, 1994
- An Osmosensing Signal Transduction Pathway in YeastScience, 1993
- STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast.Genes & Development, 1990
- Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseGene, 1988
- Recovery of S. cerevisiae a cells from G1 arrest by α factor pheromone requires endopeptidase actionCell, 1979