Partial Purification and Some Enzymatic Properties of a Proteinase from Aeromonas proteolytica
- 1 June 1969
- journal article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 131 (2) , 382-387
- https://doi.org/10.3181/00379727-131-33884
Abstract
A proteinase possessing the characteristics of an endopeptidase was partially purified from culture filtrates of Aeromonas proteolytica. The enzyme readily hydrolyzed hemoglobin, casein, insulin, CGPN, CGPEE, and CGP; with the latter substrate Km was 4.19 × 10−2 M. Chelating agents or dialysis at low pH inhibited the proteinase, which could be reactivated under favorable conditions by the addition of certain divalent metal ions.This publication has 0 references indexed in Scilit: