Stem Cell Mobilization in Normal Donors

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Abstract
We studied peripheral blood and apheresis samples from 39 consecutive normal donors who were parents or siblings of patients who received matched or mismatched bone marrow transplants using a combination of rhG-CSF-mobilized peripheral blood stem cells (PBSCs) and bone marrow (BM). BM was harvested from donors 1–7 days before starting rhG-CSF treatment: 12 μg/kg/day rhG-CSF was administered by continuous s.c. infusion for 4–7 days. Peripheral blood progenitor cells were harvested by leukapheresis using an automated continuous-flow blood cell separator, beginning on day 4 of rhG/CSF, for 1–4 consecutive days. Peak peripheral blood CD34+ cell and CFU-GM levels were reached simultaneously on day 5 or 6 of rhG-CSF administration. Median peak levels were 1.65% for CD34+ cells (range 0.34%–4.7%) and 142 CFU-GM/105 plated cells (range 16–700). The greatest numbers of CD34+ cells and CFU-GM, expressed per liter of blood volume processed, were harvested during the second and third leukaphereses: CD34+ cells 37.77 ± 25.48 × 106 and CFU-GM 3.32 ± 2.51 × 106 during the second leukapheresis, and CD34+ cells 37.01 ± 16.33 × 106 and CFU-GM 3.82 ± 4.36 × 106 during the third. The number of CD34+ cells and CFU-GM did not correlate with the sex, age, or body weight of the donors. This study indicates that this protocol for administration of rhG-CSF mobilizes large numbers of hematopoietic progenitor cells into the peripheral blood and that bone marrow harvesting before G-CSF administration does not impair stem cell mobilization.

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