Use of Bordetella pertussis BP3385 To Establish a Cutoff Value for an IS 481 -Targeted Real-Time PCR Assay
- 1 November 2008
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 46 (11) , 3798-3799
- https://doi.org/10.1128/jcm.01551-08
Abstract
This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C(T)) values of >35. IS481 PCRs with C(T) values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.Keywords
This publication has 12 references indexed in Scilit:
- What is new in pertussis?European Journal of Pediatrics, 2007
- Comparison of culture and PCR for detection of Bordetella pertussis and Bordetella parapertussis under routine laboratory conditionsJournal of Medical Microbiology, 2004
- Bordetella Species Are Distinguished by Patterns of Substantial Gene Loss and Host AdaptationJournal of Bacteriology, 2004
- An outbreak of pertussis among young Israeli soldiersEpidemiology and Infection, 2003
- Evaluation of a real-time PCR assay for detection of Bordetella pertussis and B. parapertussis in clinical samplesJournal of Medical Microbiology, 2001
- Pertussis Infection in Fully Vaccinated Children in Day-Care Centers, IsraelEmerging Infectious Diseases, 2000
- Comparison of PCR, Culture, and Direct Fluorescent-Antibody Testing for Detection of Bordetella pertussisJournal of Clinical Microbiology, 1999
- Outcomes ofBordetellaInfections in Vaccinated Children: Effects of Bacterial Number in the Nasopharynx and Patient AgeClinical and Diagnostic Laboratory Immunology, 1999
- Evaluation of PCR for Diagnosis of Bordetella pertussis and Bordetella parapertussis InfectionsJournal of Clinical Microbiology, 1998