Retinoic acid controls expression of epidermal transglutaminase at the pre‐translational level
- 12 November 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 258 (1) , 35-38
- https://doi.org/10.1016/0014-5793(89)81609-6
Abstract
Human epidermal keratinocytes were cultured until sub-confluence in low Ca2+ (0.15 mM) serum-free synthetic MCDB 153 medium. Raising the Ca2+ concentration to 1.15 mM caused an increase in envelope competence as well as plasma membrane associated transglutaminase (TGm) activity. This increase was not observed when the high Ca2+ medium contained retinoic acid. Immunofluorescence studies as well as immunoblotting with the TGm-specific monoclonal antibody B.C1 revealed that retinoic acid inhibits expression of TGm. Isolation and in vitro translation of mRNA with subsequent immunoprecipitation showed that retinoic acid inhibits TGm expression at the pretranslational level.Keywords
This publication has 21 references indexed in Scilit:
- Transglutaminases in Normal and Transformed Human Keratinocytes in CultureJournal of Investigative Dermatology, 1988
- Identification of a second human retinoic acid receptorNature, 1988
- Localization and In Vivo Activity of Epidermal TransglutaminaseJournal of Investigative Dermatology, 1988
- Plasma membrane transglutaminase and cytosolic transglutaminase form distinct envelope-like structures in transformed human keratinocytesFEBS Letters, 1988
- A human retinoic acid receptor which belongs to the family of nuclear receptorsNature, 1987
- Plasma membrane transglutaminase and cornified envelope competence in cultured human keratinocytesFEBS Letters, 1985
- Retinoid suppression of transglutaminase activity and envelope competence in cultured human epidermal carcinoma cellsDifferentiation, 1985
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Changes in expression of albumin and .alpha.-fetoprotein genes during rat liver development and neoplasiaBiochemistry, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970