Growth of human malignant lymphoid cell lines in serum-free medium
- 1 January 1983
- journal article
- research article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology - Plant
- Vol. 19 (1) , 67-71
- https://doi.org/10.1007/bf02617996
Abstract
Human T lymphoid cell lines (MOLT-4f, MOLT-3, HSB-2, CEM) and human B lymphoid cell lines (BJAB, RAJI, WIL-2) were grown longterm (up to 8 months) in serum-free medium. This medium consisted of Iscove's modified Dulbecco's medium (IMDM), supplemented with bovine serum albumin (BSA) and transferrin (TF). This serum-free medium containing albumin and transferrin is designated AT-IMDM. Lipids were not essential. Cell viability remained high, greater than 80%, in the serum-free medium and the cells maintained their distinctive characteristics. Interleukin-2 (IL-2) production capacity was maintained by the human T lymphoid cell lines JURKAT-77 and MO in short term culture. This simple medium composed of relatively inexpensive and readily available components should be useful for studies of lymphoid cell growth and differentiation and lymphoid cell products.Keywords
This publication has 16 references indexed in Scilit:
- Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.The Journal of Experimental Medicine, 1980
- Clones of Killer and Helper T Cells: Growth Requirements, Specificity and Retention of Function in Long‐Term CultureImmunological Reviews, 1980
- Plasmacytomas, lymphomas and hybridomas: Their contribution to immunology and molecular biologyBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1980
- Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterolExperimental Cell Research, 1980
- Methods for growth of cultured cells in serum-free mediumAnalytical Biochemistry, 1980
- Replacement of serum by insulin, transferrin, albumin, phosphatidyl choline, cholesterol, and some trace elements in cultures of mouse myeloid leukemia cells sensitive to inducers of differentiationExperimental Cell Research, 1979
- The use of hormone-supplemented serum-free media in primary culturesExperimental Cell Research, 1979
- [6] The growth of cells in serum-free hormone-supplemented mediaPublished by Elsevier ,1979
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes.The Journal of Experimental Medicine, 1978
- Growth Factors in Mammalian Cell CultureAnnual Review of Biochemistry, 1976